Department of Veterinary Medicine, University of Milan, Via Celoria 10, 20133 Milan, Italy.

Azienda Socio-Sanitaria Territoriale Sette Laghi, viale Borro 57, 21100 Varese, Italy.

Canile Sanitario Agenzia di Tutela della Salute Città metropolitana Milano, via Privata Aquila 82, 20134 Milan, Italy.

The aim of the study was to determine the seroprevalence of feline panleukopenia virus (FPV), feline herpesvirus type 1 (FHV-1) and feline calicivirus (FCV) in stray colony cats from Milan, Italy. Cats were divided in groups based on age, gender, reproductive status, health status and colony of origin. Blood samples were tested with an in-clinic ELISA test. The possible presence of a link between the antibody titre or the presence of seropositive results and the independent variables (age, gender, reproductive status, health status and colony location) was assessed by means of multinomial and univariate logistic regression models, respectively. Seroprevalence of 85.4% was reported for FCV. The diffusion of the other two pathogens in the cat population was much lower compared to FCV, with 45.7% and 37.1% seroprevalence observed for FPV and FHV-1, respectively. An increase of antibody titres from kitten to senior was generally observed for the three pathogens. Age was a statistically significant variable for FHV-1, with senior cats significantly associated with higher antibody titres and higher percentages of seropositive animals compared to younger age groups. Neutered cats had significantly higher antibody titres and showed significantly higher FHV-1 seroprevalences compared to sexually intact cats. Colonies from two of the nine administrative districts of Milan showed significantly higher FPV seroprevalences compared to the others. No other significant differences were observed. Our results, based on cats belonging to 70 different colonies located in urban areas far from each other, suggest that the three viruses circulate in the feline population of stray cats in Milan. The feline calicivirus represents the most common circulating pathogen, as observed also in other studies worldwide. Finally, our results suggest that stray cats may be not adequately protected against FPV, FHV-1 and FCV and vaccination could be a possible strategic solution, especially for FPV. 中文翻译： 该研究的目的是确定意大利米兰的流浪猫的猫泛白细胞减少症病毒（FPV），猫1型疱疹病毒（FHV-1）和猫杯状病毒（FCV）的血清阳性率。根据年龄，性别，生殖状况，健康状况和起源殖民地将猫分为几组。血样通过临床ELISA测试进行测试。分别通过多项式和单变量逻辑回归模型评估了抗体效价或血清阳性结果与独立变量（年龄，性别，生殖状况，健康状况和菌落位置）之间可能存在联系的可能性。据报道，FCV的血清阳性率为85.4％。与FCV相比，猫群中其他两种病原体的扩散要低得多，FPV和FHV-1的血清阳性率为45.7％和37.1％，分别。通常观察到三种病原体的抗体滴度从小猫到高级。年龄是FHV-1的统计学显着变量，与年轻年龄组相比，高龄猫与较高的抗体滴度和较高的血清反应阳性动物百分比显着相关。与性健全的猫相比，中和的猫的抗体滴度明显更高，并且FHV-1血清阳性率也明显更高。米兰九个行政区中有两个行政区的菌落FPV血清阳性率明显高于其他地区。没有观察到其他显着差异。我们的研究结果基于猫，这些猫属于彼此远离市区的70个不同殖民地，表明这三种病毒在米兰的猫科动物中传播。猫性杯状病毒是最常见的循环病原体，在全球其他研究中也观察到。最后，我们的结果表明，流浪猫可能没有得到足够的FPV，FHV-1和FCV防护，接种疫苗可能是一种可能的战略解决方案，尤其是对于FPV。