Environmental sex determination (ESD) is common among ectothermic vertebrates. The stress axis and production of stress hormones (corticosteroids) regulates ESD in fish, but evidence of a similar influence in reptiles is sparse and conflicting. The central bearded dragon ( Pogona vitticeps ) has a system of sex determination involving the interplay between sex chromosomes (ZZ/ZW female heterogamety) and the thermal environment. High egg incubation temperatures induce sex reversal of the ZZ genotype, feminizing chromosomally male individuals. Here we show that corticosterone elevation is not associated with sex reversal in the central bearded dragon, either during embryonic development or adulthood. We also demonstrate experimentally that sex determination is not affected by corticosterone injection into the yolk. This strongly suggests that stress axis upregulation by high temperature during incubation does not cause sex reversal in P. vitticeps . Our work is in general agreement with other research in reptiles, which suggests that the stress axis does not mediate sex in reptiles with ESD. Alternative biological systems may be responsible for capturing environmental conditions during reptile development, such as cellular calcium and redox regulation or the action of temperature‐sensitive splicing factors. 中文翻译： 环境性别决定（ESD）在热脊椎动物中很普遍。应激轴和应激激素（皮质类固醇）的产生可调节鱼类的ESD，但对爬行动物具有类似影响的证据稀少且相互矛盾。中央有胡子的龙（ Pogona vitticeps ）的性别确定系统涉及性染色体（ZZ / ZW雌性异配子）和热环境之间的相互作用。较高的卵温育温度引起ZZ基因型的性逆转，使染色体上的男性个体女性化。在这里，我们表明，在胚胎发育或成年期中，皮质胡子酮的升高与中央胡子龙中的性逆转无关。我们还通过实验证明，性别决定不受蛋黄注射皮质酮的影响。这有力地表明，在孵化过程中高温导致的应力轴上调不会引起 白僵菌的 性逆转 。 。我们的工作与其他有关爬行动物的研究大体一致，这表明应激轴不会介导具有ESD的爬行动物的性别。替代性生物系统可能负责捕获爬行动物发育过程中的环境条件，例如细胞钙和氧化还原调节或温度敏感剪接因子的作用。