Figure 1.
GLP-2 (1-34)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist, GLP-2 (1-34). The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of GLP-2 (1-34) (Mean ± SD, n = 2). The EC
50
of GLP-2 (1-34) on this cell was 0.11 µM.
Notes:
1. EC
50
value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC
50
-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
The glucagon-like peptides include glucagon, GLP-1, and GLP-2 exert diverse actions on nutrient intake, gastrointestinal motility, islet hormone secretion, cell proliferation and apoptosis, as well as nutrient absorptionand assimilation. Glucagon-like peptide (GLP)-2, a product of the proglucagon gene, is expressed in enteroendocrine cells of the small and large intestine and is trophic to the gastrointestinal mucosa. GLP-2 also inhibits gastric acid secretion and emptying and up-regulates intestinal hexose transport. GLP-2 acts via binding to a single G protein-coupled GLP-2 receptor (GLP-2R).
Freeze Medium
45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)
Figure 1.
GLP-2 (1-34)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist, GLP-2 (1-34). The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of GLP-2 (1-34) (Mean ± SD, n = 2). The EC
50
of GLP-2 (1-34) on this cell was 0.11 µM.
Notes:
1. EC
50
value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC
50
-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
