Western blot analysis of immunoprecipitates from GST fusion protein lysates. GST-tagged protein was immunoprecipitated with
Rabbit Anti-GST-Tag Polyclonal Antibody (GenScript, A00097) and specificity was confirmed by immunprecipitation with rabbit IgG.
Western blot was performed using Rabbit Anti- GST-Tag Polyclonal Antibody (GenScript, A00097).

Specificity This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems. Host Species Rabbit Immunogen Recombinant Glutathione-S-Transferase from Schistosoma japonicum Species Reactivity Schistosoma japonicum Conjugate Unconjugated Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5 mg/ml. Storage Instructions The product remains stable up to 1 year at -20 ℃ from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 ℃ or for up to 12 months at -20 ℃ or below. Avoid repeated freezing and thawing cycles. Purification Affinity chromatography Isotype Rabbit IgG Clonality Polyclonal Clone ID Not applicable

Western blot analysis of immunoprecipitates from GST fusion protein lysates. GST-tagged protein was immunoprecipitated with
Rabbit Anti-GST-Tag Polyclonal Antibody (GenScript, A00097) and specificity was confirmed by immunprecipitation with rabbit IgG.
Western blot was performed using Rabbit Anti- GST-Tag Polyclonal Antibody (GenScript, A00097).

Target Background GenScript Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum . Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli . The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted. Synonyms Rabbit Anti-GST-tag pAb 1. Guilin Wang, et al. Cotton peroxisome-localized lysophospholipase counteracts the toxic effects of Verticillium dahliae NLP1 and confers wilt resistance. The plant journal: for cell and molecular biology. (2023-04)
2. Zheng X, et al. The protease activity of human ATG4B is regulated by reversible oxidative modification. Autophagy. (2020)
3. Guangwen Wang, et al. The G Protein-Coupled Receptor FFAR2 Promotes Internalization during Influenza A Virus Entry. J Virol. (2019-11)
4. Shoko Ueki, et al. Protein membrane overlay assay: a protocol to test interaction between soluble and insoluble proteins in vitro. J Vis Exp. (2011-08)
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