摘要： 目的 探讨灌服蛇床子素对大鼠正畸牙移动过程中牙周组织改建的影响。 方法 72只8周龄雄性SD大鼠随机分3组,高浓度（40 mg/kg）、低浓度（20 mg/kg）蛇床子素组和对照组。建立大鼠正畸牙移动模型,分别灌服蛇床子素溶液和等量溶剂,加力7、14、21、28 d后分批处死,获取包含上颌磨牙的上颌骨,测量第一磨牙近中移动距离。分别采用H-E、抗酒石酸酸性磷酸酶染色观察牙周组织变化及对破骨细胞进行计数,采用SPSS 20.0软件包对数据进行统计学分析。 结果 加力后7、14、21、28 d,上颌第一磨牙近中移动距离逐渐增大。加力第7天,低浓度组与对照组无显著差异（ P >0.05）,其余各时间点实验组牙移动距离均较对照组显著增加（ P <0.05）;高浓度组与低浓度组之间也有显著差异（ P <0.05）。组织学观察可见,张力侧成骨细胞出现,实验组较对照组明显。压力侧破骨细胞计数于加力第7天达到高峰,与对照组有显著差异（ P <0.05）,高浓度组较低浓度组多,且有显著差异( P <0.05);加力第14天,3组均减少,实验组仍较对照组多,有显著差异（ P <0.05）,高、低浓度组间无显著差异（ P >0.05）;21 d和28 d时继续减少,组间无显著差异（ P >0.05）。 结论 灌服蛇床子素能加快正畸牙移动,在早期阶段增加牙周组织中破骨细胞数量,加快牙周组织改建,其作用受剂量影响。

Abstract: PURPOSE: To investigate the effect of osthole on periodontal remodeling during orthodontic tooth movement (OTM) in rats. METHODS: Seventy two 8-week-old male SD rats were randomly equally divided into 3 groups: two experimental groups of osthole with low (20 mg/kg) and high (40 mg/kg) concentration and the control group. Models of OTM were routinely established. Rats in the experimental groups were respectively given osthole by intragastric administration, while rats in the control group received the same volume of solvent. The rats were sacrificed on 7th, 14th, 21st and 28th day after orthodontic treatment, and the maxilla was harvested and the distance between the first and second molar was measured in each stage. Hematoxylin-eosin (H-E) staining, tartrate resistant acid phosphatase (TRACP) staining were performed. The results were analyzed with SPSS 20.0 software package for one-way analysis of variance. RESULTS: The mesio-moving distance of the three groups successively increased gradually. On the 7th day, there was no difference between the low concentration group and the control group (P> 0.05); at other time point, the experimental groups exhibited significant differences from the control group(P< 0.05), and the high concentration group had more obviously mesio-movement than the low concentration group(P< 0.05). Histological observation showed that in the tension side, osteoblast appeared, but more apparent in the experimental groups than in the control group. In the pressure side, the number of osteoclast reached the peak at the 7th day, and much more osteoclasts were seen in the experimental groups than in the control group (P< 0.05), in high concentration group than in low concentration group (P< 0.05). The number of osteoclast decreased subsequently, but significant difference existed between the experimental groups and the control group (P< 0.05) on the 14th day. At other time points, there was no significant difference among the three groups(P> 0.05). CONCLUSIONS: Osthole could increase the number of osteoclast in periodontium and promote bone remodeling at the early stage of treatment, its effect is dose-dependence during OTM.

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